3M™ Harvest RC Chromatographic Clarifier
Rising cell densities and increasing levels of soluble and insoluble contaminants of cell cultures has been an ongoing challenge for the bioprocessing industry. If you are still using centrifuge or multi-stage depth filtration, fiber chromatographic clarification may be the answer to meet current and future needs.
3M™ Harvest RC Chromatographic Clarifier is a next-generation solution in harvest and clarification technology. It is designed as an efficient option to increase mAb yields while streamlining the clarification process by replacing the centrifuge and/or depth filtration process steps.
-
Scalability from bench to commercial production -
Predictable performance -
High product recovery -
Whole cell removal and minimize cell sheer -
Streamlined process and smaller footprint
AEX fiber chromatographic technology enables further compression of the process to allow for cost effective manufacturing solutions.
- Replaces primary and secondary depth filters and guard membranes
- Removes cells, cell debris, soluble and insoluble contaminates in one step
Experience 3M innovation from lab to production scale
From lab scale to manufacturing, we can help streamline and accelerate your workflow with the entire 3M™ Harvest RC Chromatographic Clarifier portfolio of innovative solutions that enable you to condense steps and improve process efficiencies.
-
Discovery phase solutionsStart your discovery process with lab scale solutions that enable you to do your job in less time with less complexity.
-
Production scale solutionsAdvance from lab to production scale with the 3M™ Harvest RC Chromatographic Clarifier portfolio of lab and manufacturing capsules.
-
Speed up the discovery process with 3M™ Harvest RC Chromatographic Clarifier, BT500
The latest addition to the 3M™ Harvest RC Chromatographic Clarifier portfolio enables you to do your job in less time with less complexity. It’s an easy-to-use, bottle-top vacuum clarifier that delivers high product recoveries and DNA reduction (<500ppb*) through a streamlined workflow.
-
Fast-processing time (< 10 min.)*
*Based on 5-8% PCV culture.
-
Single-step clarification for ideal scalability
-
Streamline upstream and downstream processes
-
Simpler process procedure compared to traditional lab approaches
Discover the benefits of 3M™ Harvest RC Chromatographic Clarifier, BT500 in your lab
-
High quality samples in less than 10 minutes*
Compare the speed of clarification in three different approaches to lab harvesting.
*Based on 5-8% PCV culture. -
See what single-step clarification looks like
Watch how simple it can be to get started.
3M science for the unique needs of the discovery phase
Streamline your workflow in the discovery phase with our lineup of lab scaling products, including 3M™ Harvest RC Chromatographic Clarifier, WP6; 3M™ Harvest RC Chromatographic Clarifier, CT15 and 3M™ Harvest RC Chromatographic Clarifier, BT500.
-
3M™ Harvest RC Chromatographic Clarifier, WP6- Well plate
- Liquid-handling robotic system compatible
- High purity
- Simple operation
-
3M™ Harvest RC Chromatographic Clarifier, CT15- Conical tube
- High-throughput screening
- High purity
- High product recovery
-
3M™ Harvest RC Chromatographic Clarifier, BT500- Bottle-top vacuum clarifier
- Lab-scale single-use chromatographic clarifier
- Rapid harvesting solution
- Delivers high-quality samples for downstream processing
-
Enables you to do your job in less time with less complexity
See more about 3M™ Harvest RC Chromatographic Clarifiers for the lab.
Discover how your lab can benefit by integrating both speed and efficiency into your process from the start. Learn more about 3M™ Harvest RC Chromatographic Clarifier lab scale solutions.
Robust scalability with predictable performance across scales
Fibrous chromatographic clarification assures scalable performance from discovery to manufacturing which allows for faster speed to clinic and market. Performance is consistent from laboratory capsules (BC4 and BC25), to scale-up capsules (BC340 and BC1020), to production capsules (BC2300 and BC16000).
Biopharmaceutical industry insights
-
See what the industry says about 3M™ Harvest RC Chromatographic ClarifierDuring a Frost & Sullivan Virtual Think Tank Early Access Series, respondents made it evident that they believe adopting 3M™ Harvest RC Chromatographic Clarifier in commercial manufacturing will eliminate key challenges in the harvest process. The multiple benefits of this single-stage, single-use chromatographic clarification solution has driven its industry adoption.
-
Read our recent co-publication with AmbrxThe use of the 3M™Harvest RC Chromatographic Clarifier, a single-stage chromatographic clarification product, is identified as an alternative strategy to a three-stage filtration train. Using bioprocess cost modeling, the overall cost of the clarification process was reduced by 17%–30% through process compression, depending on bioreactor scale. The benefits of the single-stage harvest process extended through the downstream process, resulting in a 25% relative increase in cumulative yield with comparative impurity clearance.
The next-generation solution of harvest and clarification technology to increase mAb yields while streamlining the clarification process.
Which technology is right for your process?
-
3M™ Harvest RC Chromatographic Clarifier
Technology Platforms
- Quaternary amine (Q) functionalized polypropylene nonwoven
- 0.2 μm polyether sulfone (PES) membrane
Mechanism
AEXMaterials
SyntheticFeed Stream
Direct harvestScalability
Linear scalability from lab to multi-capsule commercial productionApplications
- Direct harvest in a single stage
- Replacement of primary, secondary and guard filters
Features
- High mAb product recovery (Capsules: >95%; Conical Tube and Well Plate >90%)
- Consistent cell loading capacity
- Turbidity reduction (<15NTU)
- DNA reduction (<500 ppb)
- Cell shear minimization
- 0.1 & 0.2 μm sterile filter protection
-
3M™ Emphaze™ AEX Hybrid Purifier
Technology Platforms
- Quaternary amine (Q) functionalized polypropylene nonwoven
- Highly asymmetric 0.2 μm polyamide membrane
Mechanism
AEXMaterials
SyntheticFeed Stream
Post depth filter, post centrifugeScalability
Linear scalability from lab to multi-capsule commercial productionApplications
- Replacement of secondary depth filters in harvest and clarification
- Guard filter to Protein A
- Bioburden control
Features
- Nominal 20-40% HCP and greater than 4 logs DNA reduction
- Consistent output turbidity (<5NTU)
- Typically, ≥ 4 LRV endotoxin reduction at low conductivity and neutral pH depending on buffer
Want to simplify your process?
Innovation in cell culture production has created additional complexities for the clarification process. 3M offers single-stage and single-use solutions to help simplify your process and increase manufacturing efficiency.
Ask an expert
Fill out this form for any technical or process-related question, and an appropriate 3M Biopharmaceutical representative will contact you to answer your question or direct your request.
Find 3M biopharmaceutical solutions
-
Find solutions for process-related impurities leveraging membrane and chromatography technology.
-
Find solutions for more efficient bioprocessing across our portfolio.
Explore applications
-
One step can transform your mAb and recombinant protein manufacturing process. At 3M, we’re helping build better, more efficient and simpler manufacturing processes by developing solutions to enhance biologic drug recovery that leads to improved process economics.
-
Gene therapyGene therapy is a fast-changing, promising field with new challenges for manufacturing and separation. Learn how we can help with our technologies and expertise.
-
Plasma fractionationPlasma fractionation has existed for decades, but additional separation methods are becoming increasingly common. We offer a range of products to help you with your process.
-
VaccinesGetting the most out of your manufacturing process with speed and on budget is an increasing challenge. Our solutions may help you develop manufacturing processes for vaccines to help maximize purity, leading to overall process economics.
-
If you need solutions for catalyst removal and recovery, color removal, particulate filtration and fill-finish, 3M offers a wide range of adsorptive depth filters and membrane products for application in the chemical pharmaceutical manufacturing.
Our apologies...
An error has occurred while submitting. Please try again later...
Thank you.
Your form was submitted successfully
Our apologies.
An error has occurred while submitting. Please try again later.
Thank you!
Your form was submitted successfully
